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. 2010 Aug 31;1(4):e00191-10. doi: 10.1128/mBio.00191-10

FIG 4 .

FIG 4

Capsule enhances GAS serotype M1T1 survival within extracellular traps and resistance to human cathelicidin LL-37. (A) Immunofluorescence microscopic overlay images of viable bacteria (green) versus dead bacteria (red) entrapped within DAPI-stained NETs (blue) for WT GAS serotype M1T1 strain 5448, capsule-deficient mutant strain 5448ΔhasA, and revertant strain 5448ΔhasA.R1. Fluorescent images are representative of three independent experiments. (B) MICs for cathelicidin antimicrobial peptide LL-37 against WT GAS serotype M1T1 strain 5448, capsule-deficient mutant 5448ΔhasA, and revertant 5448ΔhasA.R1 in logarithmic and stationary growth phases. (C) Killing kinetics for WT GAS serotype M1T1 strain 5448, capsule-deficient mutant 5448ΔhasA, and revertant 5448ΔhasA.R1 in the presence of LL-37 (64 µM). The growth index was calculated as the number of CFU at the specified time point/number of CFU in the initial inoculum. Values denote arithmetic means ± SEs (error bars) and are representative of two independent experiments performed in triplicate. Values that are statistically significantly different (P < 0.05) from the value for strain 5448 are indicated by an asterisk.