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. 2010 Jun 3;285(36):27545–27552. doi: 10.1074/jbc.M110.105767

FIGURE 2.

FIGURE 2.

Identification of critical regions in GRIM-19 and p16 necessary for their association. A, modular representation of BLOCKS-predicted GRIM-19 structure. NΔ17, deletion of amino acids 1–17; HLH, helix-loop-helix; ABD; ATP-binding domain; TPD, tyrosine phosphorylation- like domain; SH3, Src homology 3; ♦, location of point mutations. B–D, interaction of GRIM-19 with endogenous p16 in HeLa cells. Cells were transfected with the indicated expression constructs. Lysates were analyzed for expression of the respective proteins followed by IP and WB analysis with the indicated antibodies. E, modular representation of p16 structure redrawn form published sources. Deletion of ankyrin-like repeats (ΔAR1–4) and C-terminal region (ΔC21) are indicated. F, interaction of p16 deletions with endogenous GRIM-19 in HeLa cells. Cells were transfected with the indicated expression constructs. Lysates were analyzed for expression of the respective proteins followed by IP and WB analysis with the indicated antibodies.