TABLE 2.

Effect of GRIM-19/16 combinations on tumor growth

Athymic nude mice were subcutaneously transplanted with MCF-7 cells (which lack endogenous p16) expressing the indicated gene products as in our earlier studies (45). Tumor growth was measured, and mean tumor volumes (n = 8 mice/group) at the end of the 11th week, the terminal point in this experiment, are shown. p values were determined using the Student's t-test.

Tumor	Mean tumor volume	p value
	mm3 ± S.D.
EV	386 ± 45	NDa
p16	287 ± 31	<0.005 vs vector
GRIM-19	264 ± 28	<0.003 vs vector
p16/GRIM-19	86 ± 19	<0.0001 vs vector
GRIM-19 NΔ17	347 ± 67	NSb
GRIM-19 NΔ17/p16	276 ± 26	<0.003 vs vector
p16 ΔAR4	342 ± 38	NS
p16 ΔAR4/GRIM-19	246 ± 23	<0.001 vs vector

^a ND, not determined.

^b NS, not significant.