FIGURE 1.

Blockade of GM-CSF induced DC development from bone marrow precursors by butyrate. Lin⁻ bone marrow cells were cultured with GM-CSF (25 ng/ml) in the presence or absence of butyrate (0.5 mm). On days 4 and 6, cultures were harvested and analyzed for CD11c and Gr-1 expression by flow cytometry. A, expression of CD11c and Gr-1 on cells with and without butyrate. B, quantification of butyrate effect on the development of granulocytes and DCs. C, dose-dependent effect of butyrate on the development of granulocytes and DCs.