FIGURE 5.

Role of HDACs in DC development. A, Lin⁻ bone marrow cells were cultured for 4 days in the presence or absence of GM-CSF (25 ng/ml), and then exposed for 4 h to acetate (0.5 mm), propionate (0.5 mm), butyrate (0.5 mm), or SAHA (1 μm). Cell lysates were analyzed for total and acetylated form of Lys¹²-histone H4. B, Lin⁻ bone marrow cells were cultured for 6 days with GM-CSF (25 ng/ml) in the presence or absence of SAHA (1 μm) and then analyzed by flow cytometry for dendritic cells and granulocytes.