FIGURE 6.

Suppression of PU. 1 and RelB expression in bone marrow cells by butyrate and propionate. A, bone marrow cells were cultured with GM-CSF (25 ng/ml) for 4 days and then treated with or without monocarboxylates (0.5 mm) for 6 h. Expression levels of PU.1 mRNA and RelB mRNA were assessed by real-time RT-PCR. Glyceraldehyde-3-phosphate dehydrogenase expression was used as the internal control. B, specific regions of the PU.1 and RelB promoters that are monitored by ChIP assay with primer sets P1, P2, and P3 (PU.1) and R1 and R2 (RelB). C, day 4 GM-CSF bone marrow cultures were treated with or without acetate (0.5 mm), propionate (0.5 mm), or butyrate (0.5 mm) for 6 h and then used for ChIP analysis with an antibody against acetylated histone H4 and different primer sets specific for PU.1 and RelB promoters.