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. 2010 Jul 2;285(36):27601–27608. doi: 10.1074/jbc.M110.102947

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Obligatory role of Slc5a8 in butyrate-induced blockade of DC development. Lin⁻ bone marrow cells from wild type, Slc5a8^−/−, and Gpr109a^−/− mice were cultured with GM-CSF (25 ng/ml) in the presence or absence of 50 μm butyrate. On day 6, cultures were analyzed for DCs. A, percent inhibition of DC development was calculated based on the number of DCs recovered on day 6 without treatment with butyrate. *, p < 0.02; **, p > 0.05. B, expression of Slc5a8 and Gpr109a in bone marrow cells from wild type, Slc5a8^−/−, and Gpr109a^−/− mice analyzed by RT-PCR. G3pdh, glyceraldehyde-3-phosphate dehydrogenase.