FIGURE 3.

ITC analyses of Tob-PABC interaction. A, trace of the calorimetric titrations of 29 × 10-μl aliquots of 408 μm PABC into the cell containing 39 μm TobN285 (upper panel) and the 1:1 mixture of TobN285 and Caf1 (lower panel, 29 μm each). B, upper panel, trace of the calorimetric titrations of 29 × 10-μl aliquots of 550 μm PABC into the cell containing 37 μm Tob(147–285); lower panel, the integrated binding isotherm obtained from the experiments. The buffer used in the ITC experiments is comprised of 10 mm NaH₂PO₄ and 50 mm NaCl (pH 7.0). The thermodynamic parameters are summarized in Table 2.