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Schematic drawing of the interactions between the Tob-Caf1 complex and the PABPC1-bound poly(A). A, dual interactions between PAM2-C of Tob and PABC and between Caf1 and the 3′-end of poly(A). The apparent Kd for the interaction of the Tob-Caf1 complex and the PABPC1-bound poly(A) can be calculated theoretically to be the product of Kd and Km. B, when eRF3 dominates the interaction with PABC, the apparent Kd of the Tob-Caf1 complex is decreased to the level of Km.
