FIGURE 3.
Preparation of oligosaccharides from SS-CS/DS-H. SS-CS/DS-H (1 mg) was exhaustively digested with CSase AC-I and then separated into its constituent oligosaccharides by gel filtration chromatography on a SuperdexTM Peptide column, as described under “Experimental Procedures.” Oligosaccharide peaks corresponding to di- to octasaccharides, and combined oligosaccharides larger than octasaccharides were individually pooled. Vo and Vt represent the void volume and the total volume (24 ml), respectively. The elution positions of the following standard oligosaccharides (56) are indicated by arrows: 1, oligosaccharides ≥12-mer (12-mer and larger); 2, CS-decasaccharides; 3, CS-octasaccharides; 4, CS-hexasaccharides; 5, CS-tetrasaccharides; 6, disulfated CS-disaccharides; 7, monosulfated CS-disaccharides; 8, unsulfated CS-disaccharides.