TABLE 2.
Disaccharide composition of SS-CS/DS subfractions obtained by affinity chromatography using an HGF-immobilized column
The purified SS-CS/DS preparation was loaded on an HGF-immobilized column and separated into three subfractions (unbound fraction, 0.15 m fraction, and 0.5 m fraction) by stepwise elution with 10 mm Tris-HCl buffer (pH 7.4) containing 0, 0.15, or 0.5 m NaCl, as described in the text. The proportion of these subfractions and the disaccharide composition of each subfraction were analyzed as described in the legend to Table I.
| Unsaturated disaccharide | Subfractions |
||
|---|---|---|---|
| Unbound | 0.15 m | 0.5 m | |
| mol % | |||
| ΔO: ΔHexUA-GalNAc | 35.6 | 9.9 | 5.8 |
| ΔC: ΔHexUA-GalNAc(6S) | 34.6 | 36.4 | 22.7 |
| ΔA: ΔHexUA-GalNAc(4S) | 26.4 | 42.4 | 52.6 |
| ΔD: ΔHexUA(2S)-GalNAc(6S) | 1.0 | 3.1 | 3.8 |
| ΔB: ΔHexUA(2S)-GalNAc(4S) | 0.2 | 2.0 | 9.7 |
| ΔE: ΔHexUA-GalNAc(6S,4S) | 2.2 | 6.2 | 8.4 |
| Sulfation degreea | 0.68 | 1.01 | 1.14 |
| Proportion of subfractions (%) | 7.9 | 50.5 | 41.6 |
a Sulfation degree was calculated as the average number of sulfate groups per disaccharide unit.