FIGURE 4.

Localization of Myo1b, Myo1b mutants, and PIP₂ in HeLa cells. HeLa cells were transfected with full-length Myc-tagged wild-type Myo1b (A–C), full-length Myc-tagged Myo1b K966A (D–F), or full-length Myc-tagged Myo1b R977A (G–I) and stained with anti-Myc antibody (A, D, and G; green) and anti-PIP₂ antibody (B, E, and H; red). C, F, and I are merged images of A and B, D and E, and G and H, respectively. PIP₂ staining was observed at the cell periphery and in filopodia. Wild-type Myo1b, but neither of the mutants, colocalized with PIP₂ at the periphery; however, like the wild type, some staining of filopodia was observed for R977A. Scale bars = 10 μm.