FIGURE 7.

Motor activity is required for colocalization of Myo1b with PLCδ1-PH-GFP in filopodia. Myo1b R165A (A; red) and Myo1b G390A (E; red) were enriched at the cell periphery in HeLa cells that were cotransfected with PLCδ1-PH-GFP (B and F; green). D and H show fluorescence intensity along the white lines in C and G, respectively. PLCδ1-PH-GFP localized at the cell periphery and in filopodia (B and F), but motor-dead Myo1b was not present in filopodia. C and G are merged images of A and B and of E and F, respectively. Scale bars = 10 μm.