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. 2010 Jun 22;285(36):27702–27712. doi: 10.1074/jbc.M110.108498

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Ccn2 is a Wnt-responsive gene in chondrocytes. A, Ccn2 mRNA levels in ATDC5 (D0 (osteochondro-progenitor) and D26 (hypertrophic) chondrocytes treated with Wnt3A or control-conditioned medium are shown. Data are expressed as -fold induction, determined by real time RT-PCR. B, relative levels of Ccn2 mRNA expression induced by KCl versus LiCl in D0 versus D26 ATDC5 chondrocytes are shown. C, relative levels of Ccn2 mRNA expression induced by overexpression of stabilized β-catenin are shown. D, relative levels of activity of the 0.5 kb Ccn2 proximal promoter construct in the presence or absence of Wnt3A in D0 versus D26 ATDC5 cells are shown. E, relative levels of TOPflash luciferase activity in D0 versus D26 ATDC5 chondrocytes are shown. F, levels of expression of total and p-β-catenin (targeted for degradation), p-glycogen synthase kinase (p-GSK; activated) and total glycogen synthase kinase, and Sox9 in lysates from D0 and D26 ATDC5 cells were analyzed by Western blot. G, shown are relative luciferase activity levels of Ccn2 promoter activity in the presence or absence of stabilized β-catenin in D0 and D26 ATDC5 chondrocytes. *, p < 0.05.