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. 2010 Jul 1;285(36):27737–27744. doi: 10.1074/jbc.M110.117960

FIGURE 2.

FIGURE 2.

CAST overexpression decreases amyloid-β plaque in APP/PS1 mice. A and B, brain sections from 10-month-old male APP/PS1 or APP/PS1/CAST littermate mice were co-stained with thioflavin S and 4G8 antibody to label amyloid-β plaques. Shown are representative images of thioflavin S staining (A) and higher magnifications of the boxed areas co-labeled with thioflavin S and 4G8 antibody (B). C–F, quantification of plaque area in the hippocampus, cerebral cortex, or corpus callosum (C and D) and plaque numbers in cerebral cortex (E and F). Shown are the means ± S.E. (n = 6 for APP/PS1, n = 4 for APP/PS1/CAST, in C and E from 8-month-old male mice; n = 4 for APP/PS1, n = 3 for APP/PS1/CAST, in D and F from 10-month-old male mice). Three sections were analyzed for each animal. G, levels of Aβ (sum of Aβ1–40 and Aβ1–42) in cerebral cortex of 6-month-old male mice were measured by ELISA. n = 14 for APP/PS1; n = 13 for APP/PS1/CAST. *, p < 0.05; **, p < 0.01, ANOVA with Student's t test. The scale bars represent 500 μm in A and 100 μm in B.