Skip to main content
. 2010 Jun 24;285(36):27745–27752. doi: 10.1074/jbc.M110.105080

FIGURE 2.

FIGURE 2.

Effects of transfection with mature and antisense inhibitor for miR-208 on differentiation in MC3T3-E1 cells. A, differentiation and mineralization in MC3T3-E1 cells transfected with miR-208 or antisense inhibitor for miRNA-208 were observed by ALP and Alizarin red staining after treatment with BMP-2. Negative control miR-NC (miR-NC) was designed to have no significant sequence similarity to mouse, rat, or human transcription products. B, the increased expression of miR-208 in the cells by the transfection significantly suppressed the ALP activity. The cells were treated with BMP-2 (300 ng/ml) for 3 days. The values of each group were expressed as the means ± S.E. of three separate experiments. Mean values with different letters are significantly different (p < 0.01, one-way analysis of variance followed by Fisher's multiple-range test). C, effects of miR-208 or antisense inhibitor for miRNA-208 on BMP-2-stimulated ALP activities in MC3T3-E1 cells. The cells transfected with miR-208 significantly suppressed the ALP activity following BMP-2 stimulation (panel i), but antisense inhibitor for miRNA-208 was unchanged compared with those of BMP-2-treated MC3T3-E1 cells (panel ii). The values of each group were expressed as the means ± S.E. of three separate experiments.