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Effect of the cTnC mutants on the Ca2+ dependence of actomyosin ATPase activity. A shows the Ca2+ sensitivity (pCa50) of the ATPase obtained using 1 μm cTn complex containing WT cTnC (+F27W) versus WT cTnC (−F27W). B, comparison of the ΔpCa50 values obtained from complexes containing cTnC mutants (−F27W). Data represent n = 4–8 and are reported as S.E. and shown in Table 1.
