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Receptors targeted to caveolae show slower GIRK channel activation kinetics. Representative current traces recorded from a HEK293 stable line expressing Kir3.1/Kir3.4 (GIRK1/4) transiently transfected with either M2 (upper left panel), M2-YFP-Cav2 (upper right panel), A1 (lower left panel), or A1-YFP-Cav2 (lower right panel). Agonists (carbachol and NECA, respectively) were applied for 20 s as indicated.
