FIGURE 5.

Regulation of meiotic mRNAs by Pab2 is direct and independent of the Mei2-meiRNA complex. A, real-time quantitative PCR analysis of the indicated meiotic genes in wild-type, pab2Δ, pab2Δ mei2Δ, and pab2Δ sme2Δ strains. The levels of transcripts are relative to the wild-type strain and normalized to the nda2 mRNA. The data and error bars represent the average and standard deviation from three independent experiments (*, p < 0.05; Student's t test). B, immunoprecipitation (IP) experiments showing that polyadenylated meiRNA, but not the cytoplasmic srp7 RNA, is enriched in Pab2-TAP precipitates. The meiRNA and srp7 cDNAs were synthesized using oligonucleotide d(T) and random primers, respectively. C, quantification using real-time quantitative PCR for the enrichment of meiotic RNAs in Pab2-TAP precipitates. The -fold changes are relative to an untagged control strain and normalized to the cytosolic srp7 RNA. The data and error bars represent the average and standard deviation from three independent experiments.