FIGURE 6.

KN-62 treatment decreases PTEN-pAkt binding induced by atorvastatin. Cells were treated with KN-62 (100 nm for 10 min) and thereafter with insulin (1 μg/ml for 15 min) and atorvastatin (1 μm for 3 min). Photographs show cells subjected to PLA analysis. Purple dots indicate proximity between cellular bound antibodies for PTEN and pAkt Thr³⁰⁸. Diagrams show the distribution of PLA signals between the nucleus and the cytoplasm in individual cells (each square represents a single cell). 50 cells from each treatment category were included in the analysis.