FIGURE 7.

Atorvastatin and BzATP induce nuclear localization of PCNA and binding to p21^cip1. In A and B, cells were pretreated with insulin (1 μg/ml for 15 min) and thereafter with atorvastatin (1 μm for 3 or 5 min) or with BzATP (100 μm for 5 min). In C, cells were pretreated with insulin (1 μg/ml for 15 min) and thereafter with atorvastatin (1 μm for 1–5 min) or ATP (100 μm for 1–5 min), and then the lysates were immunoprecipitated (IP) using PCNA or p21^cip1 antibodies. The samples were analyzed by Western blotting, employing antibodies for p21^cip1 and PCNA. Total lysates were analyzed for Cdk2.