TABLE 2.
Mass spectrometric identification of Lsm3 purification products
L. tarentolae proteins were identified by mass spectroscopy as described under “Experimental Procedures.” Each protein is described by the systematic GeneDB name, annotation, molecular mass (kilodaltons), T. brucei homologue, molecular mass of the T. brucei homologue, and reference. An asterisk after the annotation indicates that this protein was also identified by SmD3 purification. Proteins were grouped into Lsm proteins, Sm proteins, U5 snRNP, U4/U6 snRNP, U5 snRNP, proteins with known functions, Prp19/CDC5/CWC, helicases/ATPases, and hypothetical proteins.
| Protein category and accession no | Annotation | Molecular mass | T. brucei homologies | Molecular mass | Source or Ref. | |
|---|---|---|---|---|---|---|
| kDa | kDa | |||||
| Lsm proteins | ||||||
| 1 | LmjF16.1250 | Lsm2* | 15.8 | Tb927.8.5180 | 13.2 | 26 |
| 2 | LmjF05.0540 | Lsm3 | 14.1 | Tb927.7.7380 | 10.1 | 11 |
| 3 | LmjF32.0715 | Lsm4 | 21.1 | Tb11.01.5535 | 14.1 | 11 |
| 4 | LmjF28.0970 | Lsm5 | 17.4 | tryp_XI-1036e06.p1k.embl | 13.9 | 26 |
| 5 | LmjF25.1705 | Lsm8* | 13.8 | Tb927.3.1780 | 14 | 11 |
| Sm proteins | ||||||
| 6 | LmjF27.1970 | SmB* | 11.8 | Tb927.2.4540 | 12.3 | 60 |
| 7 | LmjF22.1060 | SmD1* | 11.2 | Tb927.7.3120 | 11.7 | 60 |
| 8 | LmjF33.3190 | SmD2* | 11.7 | Tb927.2.5850 | 12.5 | 60 |
| 9 | LmjF34.3860 | SmD3* | 12.2 | Tb927.4.890 | 12.3 | 60 |
| 10 | LmjF35.4460 | SmF* | 8.5 | Tb09.211.1695 | 8.3 | 60 |
| 11 | LmjF32.1070 | SmG* | 8.7 | Tb11.01.5915 | 8.9 | 60 |
| U5 snRNP | ||||||
| 12 | LmjF35.3950 | PRP8* | 277.4 | Tb09.211.2420 | 277.4 | 63 |
| 13 | LmjF17.0920 | U5–200K/Brr2* | 260.2 | Tb927.5.2290 | 249.2 | |
| 14 | LmjF32.2200 | U5–116K/Snu114* | 110.2 | Tb11.01.7080 | 105.4 | 21, 23 |
| 15 | LmjF32.2490 | U5–102K/PRP6* | 137.5 | Tb11.01.7330 | 111 | 63 |
| 16 | LmjF28.2960 | U5–40K* | 47.5 | Tb11.01.2940 | 35 | 21, 23 |
| 17 | LmjF23.0650 | U5–15K/Dib1* | 24.2 | Tb927.8.2560 | 17.7 | 23 |
| U4/U6 snRNP | ||||||
| 18 | LmjF01.0560 | PRP3* | 67 | Tb09.160.2900 | 63.2 | 21, 23 |
| 19 | LmjF21.1120 | PRP4* | 63.8 | Tb927.10.960 | 65.4 | 21, 23 |
| 20 | LmjF15.1470 | SNU13* | 13.5 | Tb09.160.3670 | 13.5 | 62 |
| Proteins with known functions | ||||||
| 21 | LmjF28.2770 | HSP70* | 71.6 | Tb11.01.3110 | 75.3 | |
| 22 | LmjF26.1240 | HSP70 | 70.5 | Tb927.7.710 | 70.2 | |
| 23 | LmjF16.1630 | SIK1P* | 26 | Tb927.8.4790 | 26 | 23 |
| 24 | LmjF33.0814 | β-Tubulin* | 49.7 | |||
| 25 | LmjF13.0380 | α-Tubulin* | 49.7 | |||
| 26 | LmjF17.0085 | Translation elongation factor TEF1* | 49.1 | Tb927.10.2100 | 49.1 | 21 |
| 27 | LmjF25.0670 | Epsin | 65.9 | Tb11.50.0006 | 55.2 | |
| 28 | LmjF21.0885 | Mismatch repair protein | 148.3 | Tb927.10.1270 | 127.3 | |
| 29 | LmjF28.2750 | Activated protein kinase C receptor (LACK) | 34.3 | Tb11.01.3170 | 34.6 | |
| Prp19/CDC5/CWC | ||||||
| 30 | LmjF36.4820 | PRP46* | 55.6 | Tb927.10.10170 | 48.7 | |
| Helicases/ATPases | ||||||
| 31 | LmjF35.0370 | DHH1* | 46.4 | Tb927.10.3990 | 46.4 | 66 |
| 32 | LmjF07.0340 | RNA helicase* | 55 | Tb927.8.1510 | 62.4 | |
| 33 | LmjF35.3100 | RNA helicase | 100.2 | Tb09.211.3510 | 82.7 | |
| 34 | LmjF13.0500 | RNA helicase* | 225 | Tb11.02.1930 | 248.3 | |
| 35 | LmjF32.0400 | RNA helicase* | 66.8 | Tb927.10.14550 | 71.3 | |
| Hypothetical proteins | ||||||
| 36 | LmjF32.2360 | Hyp* | 87.4 | Tb11.01.7210 | 71 | |
| 37 | LmjF25.0540 | Hyp* | 32.3 | Tb11.03.0530 | 31.2 | |
| 38 | LmjF03.0790 | Hyp | 45.4 | Tb927.3.2700 | 42.6 | |
| 39 | LmjF23.0290 | Hyp* | 37.2 | Tb927.8.2230 | 41.6 | |