TABLE 3.
Mass spectrometric identification of U1A purification products
L. tarentolae proteins were identified by mass spectroscopy as described under “Experimental Procedures.” Each protein is described by the systematic GeneDB name, annotation, molecular mass (kilodaltons), T. brucei homologue, molecular mass of the T. brucei homologue, and reference. An asterisk after the annotation indicates that this protein was also identified by CPSF73 purification. Proteins were grouped U1 snRNP, RNA processing factors, helicases/ATPases, chaperons, translation factors, post-translation factors, and other proteins.
| Protein category and accession no. | Annotation | Molecular mass | T. brucei homologue | Molecular mass | Source or Ref. | |
|---|---|---|---|---|---|---|
| kDa | kDa | |||||
| U1snRNP | ||||||
| 1 | LmjF16.1590 | U1–70K | 31.5 | Tb927.8.4830 | 31.7 | 8 |
| 2 | LmjF36.6985 | U1A | 17.9 | Tb927.10.8300 | 18 | 23 |
| Tb927.10.8280 | 16 | |||||
| 3 | LmjF21.0320 | U1C | 25.1 | Tb927.10.2120 | 21.7 | 8 |
| 4 | LmjF27.1970 | SmB | 11.8 | Tb927.2.4540 | 12.3 | 60 |
| 5 | LmjF22.1060 | SmD1 | 11.2 | Tb927.7.3120 | 11.7 | 60 |
| 6 | LmjF33.3190 | SmD2* | 11.7 | Tb927.2.5850 | 12.5 | 60 |
| 7 | LmjF34.3860 | SmD3 | 12.2 | Tb927.4.890 | 12.3 | 60 |
| 8 | LmjF30.1205 | SmE | 9.8 | Tb927.6.2700 | 9.6 | 60, 61 |
| 9 | LmjF35.4460 | SmF | 8.5 | Tb09.211.1695 | 8.3 | 60 |
| RNA processing factors | ||||||
| 10 | LmjF28.2960 | U5–40K | 47.5 | Tb11.01.2940 | 35 | 21, 23 |
| 11 | LmjF27.2480 | PRP19 | 53.1 | Tb927.2.5240 | 54.2 | 21, 23 |
| 12 | LmjF17.0710 | CEF1 | 89 | Tb927.5.2060 | 80.1 | |
| 13 | LmjF35.5040 | Poly(A)-binding protein 1/PABP1 (Cytoplasma) | 62.6 | Tb09.211.0930 | 63 | |
| 15 | LmjF33.1150 | Pumilio/PUF RNA-binding protein 6* | 93 | Tb927.10.11760 | 93 | |
| 16 | LmjF36.5100 | Pumillio/PUF RNA-binding protein 11 | 105.3 | Tb11.01.2580 | 99.3 | |
| 17 | LmjF34.3430 | CPSF73* | 84.1 | Tb927.4.1340, | 85.3 | |
| 18 | LmjF35.2330 | Importin β2 subunit | 99.4 | Tb09.211.4360 | 103.4 | |
| Helicases/ATPases | ||||||
| 19 | LmjF32.0400 | RNA helicase* | 66.8 | Tb927.10.14550 | 71.3 | |
| 20 | LmjF35.0370 | DHH1* | 46.4 | Tb927.10.3990 | 46.4 | 66 |
| 21 | LmjF07.0340 | RNA helicase* | 55 | Tb927.8.1510 | 62.4 | |
| 22 | LmjF35.3100 | RNA helicase | 100.2 | Tb09.211.3510 | 82.7 | |
| 23 | LmjF21.1552 | RNA helicase* | 49.4 | Tb927.10.540 | 49.2 | |
| 24 | LmjF05.0510 | ATPase alpha subunit* | 62.5 | Tb927.7.7420 | 63.5 | |
| Tb927.7.7430 | ||||||
| Chaperons | ||||||
| 25 | LmjF23.1220 | T-complex protein 1, γ subunit* | 60.3 | Tb927.8.3150 | 60.8 | |
| 26 | LmjF36.6910 | Chaperonin, putative, T-complex protein 1 (θ subunit)* | 58.1 | Tb927.10.8190 | 58 | |
| Translation factors | ||||||
| 27 | LmjF17.0085 | Translation elongation factor TEF1* | 49.1 | Tb927.10.2100 | 49.1 | 21 |
| 28 | LmjF09.1070 | Eukaryotic translation initiation factor 2 subunit* | 52.6 | Tb11.01.4830 | 52 | |
| 29 | LmjF33.2740 | Translation initiation factor IF-2* | 92.4 | Tb927.2.3780 | 94.4 | |
| Post-translation factors | ||||||
| 30 | LmjF36.3530 | Polyubiquitin* | 94 | Tb11.01.1680 | 76.6 | |
| 31 | LmjF31.2280 | ADP-ribosylation factor* | 20.5 | |||
| 32 | LmjF04.0480 | ADP-ribosylation factor, putative | 21 | Tb09.160.5600 | 21.2 | |
| 33 | LmjF29.0880 | ADP ribosylation factor 3, putative* | 20.1 | Tb927.3.3450 | 19.8 | |
| 34 | LmjF05.0030 | Ras-like small GTPases* | 21.7 | Tb927.5.4500 | 21.7 | |
| 35 | LmjF29.0120 | Proteasome regulatory non-ATPase subunit* | 99.3 | Tb927.3.5520 | 99.9 | |
| 36 | LmjF28.1730 | Proteasome regulatory non-ATPase subunit 2* | 106 | Tb11.01.0960 | 106.5 | |
| 37 | LmjF22.0570 | Proteasome regulatory ATPase subunit 1* | 49 | Tb927.7.2500 | 48.4 | |
| 38 | LmjF13.1360 | Hyp* (alkaline phosphatase) | 90.9 | Tb11.02.0980 | 87.8 | |
| Other proteins | ||||||
| 39 | LmjF25.1420 | GTP-binding protein* | 24.2 | Tb927.3.1120 | 24.4 | |
| 40 | LmjF05.0280 | Protein-tyrosine phosphatase* | 25.1 | Tb927.10.10610 | 25.4 | |
| 41 | LmjF23.0290 | HUS1-LIKE/Hyp* | 37.2 | Tb927.8.2230 | 41.6 | |
| 42 | LmjF21.0820 | Hyp | 149.7 | Tb927.10.1490 | 134.8 | |
| 43 | LmjF32.3210 | Hyp* | 24.2 | Tb11.01.8590 | 24.4 | |
| 44 | LmjF33.0814 | β-Tubulin* | 49.7 | |||
| 45 | LmjF13.0380 | α-Tubulin* | 49.7 | |||