FIGURE 1.

SDS-PAGE analysis of the glycosylated (lanes 1 and 3) and deglycosylated (lanes 2 and 4) purified ScAGal. Glycosylated protein migrates as a broad band with an average molecular mass of 80 kDa (lane 1). Deglycosylated protein has a molecular mass of 52 kDa as predicted from protein sequence (lane 2). The second band in lane 2, which corresponds to endoglycosidase H, was removed in the last step of purification. In lanes 3 and 4, denaturing and reducing agents were avoided to perform a semi-native PAGE where oligomers are not disrupted.