FIGURE 8.

Phylogenetic analysis of GH27 family. The enzymes are clustered in five groups colored differently. Those members with known structure are highlighted in a box, and their foldings are given with the same color code as the groups to which they belong. Relevant loops common to all the enzymes in a cluster are represented and labeled. Protein sequences from enzymes classified in CAZy family GH27 as α-galactosidases (EC 3.2.1.22) or α-N-acetyl-galactosaminidases (EC 3.2.1.49) and also some noncharacterized protein sequences were retrieved from the UNIPROT data base and labeled with the name of the organism and the UNIPROT reference code. Not characterized protein sequences are labeled with “nc” before the name of the protein. Sequence alignment was performed with ClustalW2 (47), and then converted into a phylogenetic tree using the program Phylip Drawgram at the Pasteur Institute server (48).