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. 2010 Jun 28;285(36):28064–28075. doi: 10.1074/jbc.M109.096909

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FIGURE 3. — FOG1 SUMOylation is regulated by SENP1 and GATA factors. Constructs containing either a GFP-SUMO1 fusion protein or a GFP-SUMO1 fusion in which a G97A mutation (to abrogate SUMO1 attachment) was engineered were co-expressed in 293T cells with a construct containing FLAG-Bio-tagged FOG1. After immunoprecipitation (IP) was performed on whole cell lysates with a FOG1 antibody, input and immunoprecipitates were run for Western blot with anti-FLAG or anti-GFP (A). A construct containing a GFP-SUMO1 fusion protein was co-expressed with a construct containing FLAG-Bio-tagged FOG1 in the presence or absence of the FLAG-tagged SUMO-isopeptidase SENP1. After immunoprecipitation was performed on whole cell lysates with a FOG1 antibody, input and immunoprecipitates were run for Western blot with anti-FLAG and anti-GFP (B). A construct containing a GFP-SUMO1 fusion protein was co-expressed with a construct containing HA-tagged FOG1 alone or with FLAG-tagged GATA1 or FLAG-tagged GATA2. After immunoprecipitation was performed on whole cell lysates with a FOG1 antibody, input and immunoprecipitates were run for Western blot with anti-HA, anti-FLAG, and anti-GFP (C).