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Transcriptional analyses. S. reticuli wild-type (WT), hbpS disruption mutant (Δh), and senS-senR disruption mutant (Δs-r) were cultivated in the absence (−) or presence (+) of FeCl2 previously treated with DTT. After isolation of total RNAs, RT-PCRs were performed to amplify cDNAs corresponding to cpeB (cpeB-cDNA), hbpS (hbpS-cDNA) and the gene for the 16 S rRNA (16s rRNA-cDNA). 5 μl of each RT-PCR product were electrophoresed on an agarose gel.
