FIGURE 6.
Expression of the NF-κB-dependent anti-apoptotic genes is regulated by AKIP1. 293 cells, transfected with AKIP1 or control vehicle vector, were treated with Bt2cAMP for 12 h and TNFα for an additional 6 h. The total RNA was prepared from cells, and cDNA was reverse-transcribed using 0.3 μg of total RNA of each sample. A–E, real time-PCR was performed in triplicate to analyze the relative expression levels of anti-apoptotic genes (cyclin D1, bcl-2, bcl-xL, survivin, and xiap); gene expression is expressed relative to β-actin (mean ± S.E. presented). F, semi-quantitative reverse transcription-PCR was performed with 0.3 μg for each sample.