TABLE 1.
Effects of Aln, DMAb, Aln plus PTH, or DMAb plus PTH on bone mass and vertebral and femoral microarchitecture in huRANKL mice
In parentheses are the number of mice per group. Conn dens, connectivity density.
| Sham (6) | OVX |
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|---|---|---|---|---|---|---|---|
| No PTH |
PTH |
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| Veh (6) | Aln (8) | DMAb (8) | Veh (6) | Aln (7) | DMAb (8) | ||
| BMD change (%, week 8-week 0) | |||||||
| Total body | −1.0 ± 1.2 | −4.1 ± 1.9 | 2.9 ± 1.0b | 8.1 ± 1.1b,c | 1.0 ± 0.8d | 9.2 ± 1.2d,f | 5.2 ± 1.9f,g |
| Lumbar spine | −6.8 ± 3.1 | −12.5 ± 5.6 | 7.6 ± 4.0b | 7.8 ± 4.5b | −12.1 ± 2.5 | 11.6 ± 4.3f | 6.3 ± 4.1f |
| Femoral shaft | −3.2 ± 1.3 | −1.6 ± 2.8 | 3.4 ± 1.9 | 5.5 ± 1.8b | −6.1 ± 1.1 | 4.1 ± 2.0f | 4.8 ± 3.6f |
| Vertebral trabecular bone | |||||||
| BV/TV (%) | 21.8 ± 1.6 | 15.7 ± 1.3a | 22.6 ± 0.7b | 23.7 ± 1.6b | 18.7 ± 1.2 | 28.8 ± 1.8d,f | 24.1 ± 1.9f,g |
| Tb N (/mm) | 3.4 ± 0.1 | 2.9 ± 0.2a | 3.6 ± 0.2b | 3.7 ± 0.2b | 3.0 ± 0.1 | 3.6 ± 0.1f | 3.6 ± 0.1f |
| Tb Th (μm) | 62.5 ± 2.9 | 55.2 ± 1.7a | 59.0 ± 0.9 | 62.5 ± 2.b | 59.0 ± 0.8 | 69.3 ± 1.5d,f | 62.9 ± 2.5g |
| Conn dens (mm−3) | 84.1 ± 4.1 | 89.0 ± 9.4 | 85.2 ± 6.4 | 97.2 ± 6.5 | 112.6 ± 15.9 | 81.3 ± 5.4f | 98.2 ± 9.7 |
| Cortical width (μm) | 95.3 ± 1.7 | 83.3 ± 2.1a | 100.3 ± 2.1b | 123.4 ± 5.8b,c | 92.1 ± 2.0 | 114.0 ± 3.5d,f | 114.5 ± 6.3f |
| Distal femur trabecular bone | |||||||
| BV/TV (%) | 5.0 ± 0.8 | 1.3 ± 0.3a | 4.4 ± 0.5 | 8.5 ± 1.4b,c | 2.3 ± 0.4 | 8.6 ± 1.4d,f | 7.0 ± 1.3f |
| Tb N (/mm) | 2.7 ± 0.2 | 1.6 ± 0.1a | 2.8 ± 0.1b | 3.9 ± 0.4b,c | 1.9 ± 0.2 | 3.6 ± 0.3d,f | 3.6 ± 0.4f |
| Tb Th (μm) | 42.4 ± 1.9 | 37.4 ± 2.3a | 38.5 ± 0.9 | 43.2 ± 1.3b,c | 42.1 ± 0.9d | 45.3 ± 0.8d | 40.8 ± 1.5g |
| Conn dens (mm−3) | 25.1 ± 6.6 | 2.5 ± 0.7 | 30.6 ± 4.6 | 66.8 ± 14.3b,c | 7.4 ± 1.6 | 72.4 ± 19.2d,f | 52.0 ± 12.3f |
| Cortical width (μm) | 133.4 ± 3.6 | 108.7 ± 5.4a | 122.3 ± 3.3b | 131.8 ± 5.4b | 117.4 ± 3.4 | 126.2 ± 3.2 | 129.4 ± 4.7f |
| Femoral cortical bone | |||||||
| Cross sectional area (mm2) | 0.272 ± 0.005 | 0.260 ± 0.006 | 0.279 ± 0.008 | 0.283 ± 0.014 | 0.279 ± 0.007 | 0.286 ± 0.006 | 0.272 ± 0.007 |
| Bone area (mm2) | 0.164 ± 0.002 | 0.140 ± 0.002a | 0.160 ± 0.005b | 0.153 ± 0.003b | 0.151 ± 0.003d | 0.167 ± 0.004f | 0.157 ± 0.003 |
| Medullary area (mm2) | 0.108 ± 0.003 | 0.120 ± 0.005 | 0.119 ± 0.005 | 0.130 ± 0.013 | 0.128 ± 0.005 | 0.119 ± 0.003 | 0.114 ± 0.005 |
| Cortical width (μm) | 274 ± 1 | 239 ± 4a | 262 ± 7b | 251 ± 5 | 246 ± 4 | 267 ± 6f | 262 ± 5f |
a p < 0.05 compared with sham.
b p < 0.05 compared with OVX Veh.
c p < 0.05 compared with OVX Aln.
d p < 0.05 compared with non-PTH in the same pretreatment group.
f p < 0.05 compared with OVX Veh-PTH.
g p < 0.05 compared with OVX Aln-PTH.