FIGURE 3.
Incision activity of OGG1 and NEIL1 are not significantly changed after glutamate treatment. The 8-oxoguanine and 5-hydroxycytosine lesions are mainly produced by oxidation and predominately removed by OGG1 and NEIL1, respectively. The results of biochemical and immunoblotting assays for incision activities of OGG1 (A) and NEIL1 (B) (upper panels; *, 32P-labeled 5′-end; the filled circle marks the 8-OxoG and 5-OHC lesion sites) were not affected by a 10-min pulse glutamate treatment. Values for treated cultures are expressed as a percentage of the value for untreated control cultures (100%).