TABLE 1.
Experimental interspin distance r |
Calculated interspin distance r |
|||||
---|---|---|---|---|---|---|
−K+ | +K+ | Model 1 | Model 2 | Model 3 | Model 4 | |
nm | nm | |||||
Asp-222R1/Thr-318R1 | ≥1.8a | 1.4 ± 0.1 | 2.1 and 2.4 | 1.9 | 1.8 | 1.5 |
Asp-222R1/Ser-327R1 | >1.8 | >1.8 | 1.5 | 0.9 and 1.25 | 1.7 | 1.5 and 2.0 |
Met-311R1/Thr-318R1 | 3.5 ± 0.2 | 3.5 ± 0.2 | 2.3 | 2.0 | 2.9 and 3.7 | 3.0 |
Thr-318R1/Val-331R1 | 1.5 ± 0.1 | 1.7 ± 0.2 | 2.4 and 2.9 | 2.9 | 3.2 | 1.7 |
Ser-327R1/Val-331R1 | 1.1 ± 0.1 | 1.1 ± 0.1 | 0.8 | 1.8 | 1.0 | 0.6 and 1.0 |
a The fraction of the singly spin-labeled component is in the range of 30–50%.