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Alignment of ClosC in C. botulinum ATCC 3503 and MoeB/ThiF and proposed mechanisms. A, mechanisms for the formation of thiazole or methyloxazole. B, sequence alignments of ClosC, MccB, E. coli MoeB, and E. coli ThiF. Blue highlighted boxes show conserved ATP binding residues determined by the crystal structures of ThiF and MoeB. Green highlighted boxes show the residues involved in zinc-tetrathiolate formation.
