Figure 7.

Overexpression of Hsp23 in the CNS and PNS. (A) Western blot showing the increase in Hsp23 protein level in flies carrying the actin-GAL4 chromosome in conjunction with the UAS-hsp23/4 insertion. For control purposes, levels of Hsp23 in heat-shocked S2 cells and in flies carrying the same UAS chromosome with the CyO balancer are also shown. (B) Stage 16 embryos of the elav-GAL4;UAS-hsp23/4 genotype. Cellular co-localization of Hsp23 (red) and Elav (green) in embryonic neurons. Note that perfect merging of the two signals is not obtained as the proteins differ in their intracellular localization (Elav: nuclear, Hsp23: cytoplasmic). Left inset shows ventro-lateral view with strong Hsp23 expression in the nerve cord. Right inset is a high magnification ventro-lateral view of the nerve cord showing unaffected separation of the transversal commissures (green, visualized with BP102) and general Hsp23 expression (red).