FIGURE 8.

Experimental time domain data and corresponding distance distributions. A, spin labels at positions 50 and 72 in αS PD mutants A30P, E46K, and A53T; B, spin labels at positions 24 and 61 in PD mutants A30P, E46K, and A53T; and C, spin labels at positions 24 and 83 in WT αS and the three PD mutants. Distances were reconstructed by the L-curve method. The proteins were reconstituted in DMPC/DMPG liposomes. The total protein concentration was 30 μm. In the case of proteins with spin-labeled sites 24 and 83, 70% deuterated proteins were reconstituted into DMPG-d54, DMPC-d67 proteoliposomes using deuterated buffers.