FIGURE 2.
Average fluorescence lifetime ratios between samples in detergent and under conditions favoring aggregation. A, fluorescence average lifetimes of monomeric Lhcb4 to -6, trimeric LHCII proteins and of a mixture of Lhcb6 and LHCII trimers (3:1 molar ratio) in detergent (τAV UNQ) or under aggregation conditions (τAV Q). Time-resolved fluorescence analysis is reported in Table 1. Lhcb4 to -6 and LHCII trimer average fluorescence lifetimes were measured either in violaxanthin binding (Vio) or zeaxanthin binding (Zea) samples; calculated fluorescence average lifetimes of the LHCII and Lhcb6 mixture are reported (theor). B, average fluorescence lifetime ratios among samples measured in detergent (τAV UNQ) or in aggregation conditions (τAV Q), showing actual and calculated fluorescence average lifetimes of the LHCII and Lhc6 mixture under aggregation conditions.