Figure 1.

Characterization of CCN5 RNAi in VSMC. A. VSMC transfected with fluorescein labeled siRNA and viewed live on an inverted fluorescent microscope to determine transfection efficiency. B. Q-PCR results: Total cellular RNA was isolated from control and CCN5 RNAi treated VSMC. After reverse transcription they were subjected to Q-PCR analysis to assay CCN5 message level. Graph is representative of relative CCN5 levels in the various conditions. * indicates p < 0.05 with respect to the value for expression in growth arrested cells. (AU = Arbitrary Units) C. Western blots depicting reduced CCN5 protein expression in CCN5 siRNA treated VSMC. Top panel: Blot from cell lysates probed with anti-CCN5. Bottom Panel: Same blot stripped and reprobed with anti-GAPDH to insure equal loading. Lane1: untreated lysate Lane 2: control siRNA lysate Lane 3: CCN5 siRNA lysate.