FIGURE 5.

Activities of C-terminal TFPI-derived peptides. A, antibacterial effects of the TFPI-derived peptides and LL-37 against E. coli or P. aeruginosa in viable count assays. 2 × 10⁶ cfu/ml bacteria were incubated in 50 μl with peptides at the indicated concentrations in 10 mm Tris, 0.15 m NaCl, pH 7.4 (buffer), or in 0.15 m NaCl, 10 mm Tris, pH 7.4, containing 20% human citrate plasma (CP), or in the same buffer but with heat-inactivated human citrate plasma (HCP) (n = 3, S.D. is indicated). B, the indicated bacterial isolates were subjected to GGL27 at 3 μm in buffer, native plasma (CP), or heat-inactivated plasma (HCP) for 2 h, and the number of cfu was determined.