Fig. 4.

Mammary tumors develop faster in the ST3Gal-I/PyMT mice compared to the controls. (A) Kinetics of tumor formation in ST3Gal-I/PyMT and Control/PyMT mice. The difference between ST3Gal-I/PyMT and control mice was highly significant (P < 0.001), evaluated by the Breslow test. (B) Sera from control/PyMT mice (n = 6) were taken from the same mice at days 30 and 60 after birth and assayed for TGFβ. TGFβ levels increased significantly over time and were associated with the appearance and the number of mammary tumors. (C) Sera taken from ST3Gal-I/PyMT (n = 21) and Control/PyMT (n = 14) mice at a mean time of 38 and 42 days from birth, respectively, were assayed for TGFβ using a commercial ELISA kit. The difference between groups was significant (P < 0.001) evaluated by two-tailed T test