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Increased ST3Gal activity does not affect the interaction of Muc1 with c-Src or downstream signaling. (A) Tumor lysates (1 mg) from Control/PyMT (n = 3) and ST3Gal-I/PyMT (n = 3) mice were immunoprecipitated with anti-c-Src or anti Muc1 (CT2) and immunoblotted for c-Src (upper panels) or Muc1 (CT1) (lower panels). (B) Tumor lysates (30 μg) from Control/PyMT mice (n = 4) and ST3Gal-I/PyMT mice (n = 4) were immunoblotted for phosphorylated AKT (upper panel) or total AKT (lower panel)
