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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Microbes Infect. 2010 May 11;12(10):716–726. doi: 10.1016/j.micinf.2010.04.014

Fig. 1. T. cruzi trans-sialidase proteins interact with TcPKAc.

Fig. 1

Using a yeast two-hybrid system six members of the trans-sialidase super family target plasmids interacting with pBD- TcPKAc were obtained. Four ORFs of these genes and two partial genes were subcloned into a pAD-Gal4 vector. Each pAD-Gal4 construct was co-transformed into yeast with pBD-TcPKAc and cultured in high stringency condition (-Leu, -Trp, -His)

(1) trans-sialidase Tc00. 1047053508563.20;

(2) trans-sialidase Tc00.1047053509187.10;

(3) trans-sialidase-like protein SA85-1.1 Genbank accession number X53545;

(4) trans-sialidase Tc00.1047053507839.40;

(5) trans-sialidase Tc00.104753510111.20;

(6) trans-sialidase active clone lacking SAPA repeats TCTS 611/2.

(7) Positive control (pADwt + pBDwt);

(8) Negative control (pADwt + pLaminC).