Using a yeast two-hybrid system six members of the trans-sialidase super family target plasmids interacting with pBD- TcPKAc were obtained. Four ORFs of these genes and two partial genes were subcloned into a pAD-Gal4 vector. Each pAD-Gal4 construct was co-transformed into yeast with pBD-TcPKAc and cultured in high stringency condition (-Leu, -Trp, -His)
(1) trans-sialidase Tc00. 1047053508563.20;
(2) trans-sialidase Tc00.1047053509187.10;
(3) trans-sialidase-like protein SA85-1.1 Genbank accession number X53545;
(4) trans-sialidase Tc00.1047053507839.40;
(5) trans-sialidase Tc00.104753510111.20;
(6) trans-sialidase active clone lacking SAPA repeats TCTS 611/2.
(7) Positive control (pADwt + pBDwt);
(8) Negative control (pADwt + pLaminC).