Table 1.

Table 1 A. PCR primers and annealing temperatures for trans-sialidase and TcPKAc genes
Trans-sialidase genes and TcPKAc gene	PCR primers	Annealing Temperature
Tc00.1047053508563.20	Forward- (Nhe1) CTAGCTAGC ATGCTCTCACGTGTTGCTGC Reverse- (Xbal1) GATCTCTAGA TCACGCAGTCGCAAAGCCCC	55°C
Tc00.104753509187.10	Forward- (Nhe1) CTAGCTAGCATGTCCCGGCGTGTGTTTACT Reverse- (Xbal1) GATCTCTAGATCACGCAGCCGCAAACCC	55°C
Tc00.1047053507839.40	Forward- (Xho1) CCGCTCGAG ATGCTCTCACGTGTTGCTG Reverse- (Sal1) ACGCGTCGAC TCACGCAGCCGCAAAGCCC	66°C
Tc00.104753510111.20	Forward- (Xho1) CCGCTCGAG ATGCTCTCACGTGTTGCTG Reverse- (Sal1) ACGCGTCGAC TCACGCAGTCGCAAGGCCC	66°C
TcPKAc (Genbank, AY055783)	Forward- (EcoR1) CGGAATTCATGTCAGGGGAGGCAAATTATG Reverse- (Pst1) AACTGCAGTTAGAACCCAATAAACTCCGCCTG	55°C

Table 1B. In silico analysis of PKA phosphorylation site.
Each of trans-sialidase protein sequences was analyzed against PROSITE patterns and profiles (http://us.expasy.org/prosite). All of these proteins contain typical PKA phosphorylation sites. The numbers indicate the location of the sites in the various protein sequences.
Trans-sialidase genes	cAMP- dependent protein kinase phosphorylation site
Tc00.1047053508563.20	113 – 116: KKgT; 120 – 123: RRdS; 465 – 468: RRvS; 624 – 627: KKlT
Tc00.104753509187.10	17 – 20: RRvT; 635 – 638: KKiS
Tc00.1047053507839.40	17 – 20: RRvT; 239 – 242: KRiS; 633 – 636: KKaS
Tc00.104753510111.20	17 – 20: RRvT; 468 – 471: RRgS; 649 – 652: KKrS; 699 – 702: KKpS
Tc00.1047053508285.60(SA1.1)	17 – 20: RRvT; 119 – 122: RRdS; 466 – 469: RRdS; 624 – 627: KKpT
TcTS 6 11/2	12 – 15: KRqS

Table 1C. Myristoylation sites exist in TcPKAc
The PROSITE program was used to identify N-myristoylation sequences in both TcPKAc isoforms
TcPKAc	Myristoylation site
Protein kinase A catalytic subunit isoform-1 (Tc00.1047053508461.310)	306 – 311: GLlqTD; 317 – 322: GTlkGG
Protein kinase A catalytic subunit isoform- 2 (Tc00.1047053508461.280)	25 – 30: GstlGA; 250 – 255: GLlqTD; 261 – 266:   GTlkGG