Figure 2.
Isothermal titration calorimetry of HypA proteins. The integrated heat data for titrations of apo-WT-HypA and selected mutant HypA proteins with NiCl2 solutions is shown for titrations at different pH values. The symbols represent the integrated heat after each injection, while the solid line is the calculated fit of the data used for values in Table 1 (see materials and methods).