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. 2010 Aug 4;30(31):10575–10585. doi: 10.1523/JNEUROSCI.2773-10.2010

Figure 5.

Figure 5.

EphB1 expression on motoneuron axons distal to the D-V choice point. A–C, Transverse 16 μm sections of the limb showing EphB1 expression in control (A), chronic picrotoxin (B), and light activation by ChR2 in addition to chronic picrotoxin-treated (C) embryos. EphB1 is highly expressed on the ventral nerve trunk in control embryos (A, arrow). Chronic picrotoxin treatment results in downregulation of EphB1 (B, arrow; G), while light activation by ChR2 in addition to chronic picrotoxin treatment maintains EphB1 levels (C, arrow; G) similar to control embryos. D–F, NCAM expression visualized by immunostaining with a monoclonal antibody that recognizes all isoforms of NCAM in the same sections shown in A–C. G, Mean pixel intensities of EphB1 immunostaining of the ventral nerve trunks are quantified and averaged for each condition. *p < 0.01, **p < 0.001. H, Levels of NCAM immunostaining on the ventral trunk did not differ from control for either of the treatments. Scale bar, 100 μm. Chr. Picro, Chronic picrotoxin treatment; activ, activation.