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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Nat Nanotechnol. 2010 Aug 29;5(9):676–682. doi: 10.1038/nnano.2010.160

Figure 3.

Figure 3

Structural characterization of RNA cubes by cryo-EM with single particle image reconstruction. Panels a and b correspond to the characterization of 6 and 10 stranded RNA cubes respectively. Each panel on the top left represents typical cryo-EM images of the RNA particles. On the right side, class averages for each RNA cube as observed by cryo-EM (EM) with corresponding projections of the reconstructed 3D structure and theoretical RNA cube model. Reconstructed 3D models of the six and 10-stranded RNA cubes have been obtained at 8.9 Å and 11.7 Å resolution, respectively. All RNA complexes used in cryo-EM experiments were assembled at 1 µM of each RNA strand as described in the Materials and Methods.