Simulations highlighting the effect of rotational correlation time of the fast (panel A) and intermediate (panel B) timescale motions of a ribosome-bound nascent protein on anisotropy decay data in the frequency domain. The fundamental anisotropy r0 was fixed at 0.37, and the fractional contributions of each rotation were fixed simulation parameters (FS = 0.7, FI = 0.1, FF = 0.2). In panel A, τS was fixed at 1000 ns, τI was fixed at 7 ns, and τF was varied from 0.3 ns to 2 ns. In panel B, τS was fixed at 1000 ns, τF was fixed at 0.3 ns, and τI was varied from 5 ns to 30 ns.