Fig.7. Effects of dPrx5 and JNK signaling on the immune response genes.
The genotype of the control (C) was UAS-BskDN; S106/+;+/+ and the genotype of flies under-expressing dPrx5 (dprx5) was UAS-BskDN; S106/+;dprx5/dprx5. Expression of dominant-negative Basket was achieved by feeding one week-old flies food containing 0.1 mg/ml mifeprisone dissolved in ethanol (BskDN) or ethanol-only added food as a control (−). Food with mifepristone was changed daily. A, Analysis of Basket expression. After two days of feeding mifepristone, RNA was isolated followed by real time RT-PCR analysis with primers specific for Basket. B–H, After feeding for two days food with or without mifepristone, flies were infected with 24 h broth culture of P. aeruginosa by pricking and incubated at 25°C for 0 h, 1 h and 4 h followed by RNA extraction. RT-PCR was performed with primers specific for Puckered (B), Ets21C (C), Relish (D), Dipt (E), CecC (F), AttD (G) and Drs (H) and the gene-specific signals were standardized against signals obtained for the housekeeping gene rp49. Statistically significant (P<0.05) were differences in the levels of Puc, Ets21C and Rel between control flies (no mifepristone) and flies expressing BskDN in normal (C) or dprx5 background at 1 h after infection, as denoted by asterisks. Significant differences were also in the levels of CecC between dprx5 mutant and control, expressing dominant-negative Basket at 1 h and 4 h post-infection; AttD at 4 h post-infection between control and the dprx5 mutant expressing or not expressing BskDN, as well as Drs between flies with wild type or dominant-negative Basket, as denoted by asterisks. All RT-PCR results are mean ± SD from three-four determinations for each of two independent experiments.