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. Author manuscript; available in PMC: 2011 Oct 26.
Published in final edited form as: Mol Cell Endocrinol. 2010 Jun 12;328(1-2):1–7. doi: 10.1016/j.mce.2010.05.017

Fig. 4.

Fig. 4

Production of BMP-15 by 293F cells transfected with wild-type and mutant plasmids. The 293F cells transfected with the indicated BMP-15 plasmids were cultured for the indicated hours, and cell lysates were subjected to SDS/PAGE immunoblotting analysis using anti-FLAG antibody (Ab) and anti-β-actin Ab. Note that all expression plasmids have a Flag epitope at the C-terminus. CTRL: control cells transfected with the empty vector. Results are shown as representative of those obtained from three independent experiments in panel A. The band intensity of scanned images was statistically analyzed, and the cumulative data from three independent experiments are presented with mean ± SEM in panel B. Each experiment was performed with triplicate samples. *, P <0.05; **, P < 0.01 between the indicated groups.