Table 1.

Genetic transformation of human cells by Agrobacterium

Plasmids	Host-cell type	Total number of geneticin-resistant transformants	Efficiency of transformation per 106 cells, ±SD*
pTi–pNeo in Agrobacterium	HeLa	140	17 ± 4
pTi–pNeo in Agrobacterium without preinduction	HeLa	74	9 ± 2
pNeo in Agrobacterium	HeLa	0	<0.1
pNeo alone	HeLa	0	<0.1
pNeo Ca-Phosphate	HeLa	179	19 ± 6
pTi–pNeo in Agrobacterium	HEK393 (kidney)	167	18 ± 6
pNeo in Agrobacterium	HEK393 (kidney)	0	<0.1
pNeo alone	HEK393 (kidney)	0	<0.1
pTi–pNeo in Agrobacterium	PC12 (neuronal)	112	12 ± 3
pNeo in Agrobacterium	PC12 (neuronal)	0	<0.1
pNeo alone	PC12 (neuronal)	0	<0.1

^*Microsoft excel software was used to calculate standard deviations between two to six individual experiments, each of which included 5–10 culture plates per system.