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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Cancer Res. 2010 Feb 1;70(3):948–956. doi: 10.1158/0008-5472.CAN-09-3094

Figure 4. Effect of ADAMTS1 on TSP1 cleavage by liver and lung lysates.

Figure 4

(A) Diagram of antibodies targeting TSP1. (B) Western blot of TSP1 following digestion of recombinant TSP1 (rTSP1) by normal lung or liver lysate. Also shown is rTSP1 alone (no tissue lysate) and rTSP1 incubated with recombinant ADAMTS1 (rADAMTS1). β-actin Western blot serves as loading control. (C) TSP1 Western blot examining digestion of recombinant TSP1 with lung or liver lysate, recombinant ADAMTS1 (rADAMTS1), and/or neutralizing ADAMTS1 antibody (anti-ADAMTS1). β-actin Western blot serves as loading control. (D) Western blot examining rTSP1 proteolysis by liver or lung lysates from wild-type mice (WT) and Adamts1-null mice (Adamts1-/-).