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. 2010 Jun 16;104(2):713–725. doi: 10.1152/jn.00282.2010

Fig. 7.

Fig. 7.

SICs are generated by activation of extrasynaptic NMDA receptors in postsynaptic neurons. Recordings were made at a holding potential of +40 mV in the presence of 10 μM DNQX to block non-NMDA receptors. Traces in A–C were taken from different neurons. Ai: synaptic NMDA currents (channels) were gradually blocked by stimulation repeated at 0.1 Hz at the intensity that evoked maximum NMDA EPSCs during perfusion of 40 μM MK-801 (responses to 1st, 4th, 10th, and 15th stimulations are shown). Aii: no synaptic NMDA EPSCs were evoked by the same stimulation 10 min after washout of MK-801. Aiii: 3 sweeps of recordings show 3 SICs evoked by the same stimulation intensity given every 60 s in the presence of 100 μM TBOA. Bi: synaptic NMDA currents channels were gradually blocked by stimulation repeated at 0.1 Hz at the maximum intensity with 1 mM MK-801 included in the intracellular solution (responses to 1st, 4th, 10th, and 15th stimulations are shown). Bii: no synaptic NMDA EPSCs were evoked by the same stimulation before perfusion of TBOA. Biii: in the presence of 100 μM TBOA, a novel SIC was evoked by the same stimulation but was immediately and completely blocked by MK-801 inside the recording electrode after repeated stimulations (responses to 1st, 3rd, and 7th stimulations are shown). C: after synaptic NMDA receptors were blocked by 1 mM MK-801 in the intracellular solution, a spontaneous SIC was induced by 100 μM TBOA but was immediately and completely blocked by MK-801 inside the recording electrode.